Research Paper Volume 15, Issue 22 pp 12702—12722

Chronological aging impacts abundance, function and microRNA content of extracellular vesicles produced by human epidermal keratinocytes

Figure 2. Primary keratinocytes prepared from aged skin represent a valuable model of aging. Primary keratinocytes were isolated from 3 skin biopsies from young (<5 years) and old individuals (>60 years). Cells were cultured from passage 1 (P1) to late passage 6 (P6) and protein were extracted. (A) Western-blot analysis of p16. Actin is used as a loading control. (B) Western-blot analysis of BIP/GRP78 and p63 at passage 2 (P2) and passage 6 (P6). Actin is used as a loading control.