Research Paper Volume 17, Issue 2 pp 365—392

Figure 7. Lipid peroxidation and antioxidant response in control (C1, C2) and MS (P1, P2) fibroblasts. C1 cells treated with 500 μM Luperox® (C1 + Luperox) were used as a positive control. P1 cells treated with 100 μM deferiprone (P1 + def) or 50 μM vitamin E (P1 + VitE) were used as negative controls. (A) Representative images of lipid peroxidation assessed by Bodipy® 581/591 C11 staining. Scale bar = 20 μm. (B) Quantification of oxidized form fluorescence intensity. (C) Immunoblotting analysis of antioxidant enzymes. Actin was used as the loading control. (D) Band densitometry of Western Blot data normalized to the mean of controls and referred to actin levels. Data represent the mean ± SD of three separate experiments. ***p-value < 0.0001 between control and MS fibroblasts. ^aaap-value < 0.0001 between untreated and Luperox®-treated C1 cells. ^bbbp-value < 0.0001 between untreated and deferiprone-treated P1 cells. ^cccp-value < 0.0001 between untreated and vitamin E-treated P1 cells. a.u.: arbitrary units.