Research Paper Advance Articles
Fisetin ameliorates vascular smooth muscle cell calcification via DUSP1-dependent p38 MAPK inhibition
Figure 10. Effects of fisetin ex vivo in calcifying mouse aortic explants. Alizarin Red staining (A) of mouse aortic arches cultured for 7d in medium supplemented with 1.6 mM phosphate (Pi) without and with 1 μM fisetin (Fis). Calcification: red staining; scale bar: 5 mm. Normalized calcium content (n=6, B) in mouse aortic explants cultured for 7d in medium supplemented with control (CTR) or 1.6 mM phosphate (Pi) without and with 1 μM fisetin (Fis). Relative mRNA expression (n=5) of Bmp2 (C), Cbfa1 (D), Alpl (E) and Cdkn1a (F) in mouse aortic explants cultured for 7d in medium supplemented with control (CTR) or 1.6 mM phosphate (Pi) without and with 1 μM fisetin (Fis). *(p<0.05), **(p<0.01), ***(p<0.001) significant vs. control group; †(p<0.05), ††(p<0.01), †††(p<0.001) significant vs. Pi-treated group.