Research Paper Advance Articles
Fisetin ameliorates vascular smooth muscle cell calcification via DUSP1-dependent p38 MAPK inhibition
Figure 11. Effects of fisetin in vivo during cholecalciferol overload. Alizarin Red staining (A) of aortas from mice receiving vehicle (CTR) or high-dosed cholecalciferol (vD) without and with fisetin (Fis). Calcification: red staining; scale bar: 5 mm. Normalized calcium content (n=6-9, B) in aortic tissue from mice receiving vehicle (CTR) or high-dosed cholecalciferol (vD) without and with fisetin (Fis). Relative mRNA expression (n=6-9) of Bmp2 (C), Cbfa1 (D), Alpl (E) and Cdkn1a (F) in aortic tissue from mice receiving vehicle (CTR) or high-dosed cholecalciferol (vD) without and with fisetin (Fis). *(p<0.05), **(p<0.01), ***(p<0.001) significant vs. control group; †(p<0.05), ††(p<0.01) significant vs. vD-treated group.