Research Paper Advance Articles
Fisetin ameliorates vascular smooth muscle cell calcification via DUSP1-dependent p38 MAPK inhibition
Figure 2. Effects of fisetin on pro-calcific signaling in VSMCs during pro-calcific conditions. Relative mRNA expression (n=6) of CBFA1 (A), SP7 (B), BGLAP (C) and SPP1 (D) in HAoSMCs treated for 48h with control (CTR) or calcification medium (Calc.) without and with 1 μM fisetin (Fis). (E) Normalized ALP activity (n=6) in HAoSMCs treated for 7d with control (CTR) or calcification medium (Calc.) without and with 1 μM fisetin (Fis). *(p<0.05), **(p<0.01), ***(p<0.001) significant vs. control group; †(p<0.05), ††(p<0.01), †††(p<0.001) significant vs. Calc.-treated group. (F) CBFA1 (green) and nuclei (blue) shown by confocal imaging in HAoSMCs treated for 48h with control (CTR) or calcification medium (Calc.) without and with 1 μM fisetin (Fis). Scale bar: 20 μm.