Fisetin ameliorates vascular smooth muscle cell calcification via DUSP1-dependent p38 MAPK inhibition

Mehdi Razazian; Sheyda Bahiraii; Azmat Sohail; Markus Mandl; Isratul Jannat; Georg Beilhack; Ioana Alesutan; Jakob Voelkl

doi:doi:10.18632/aging.206233

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Research Paper Advance Articles

Fisetin ameliorates vascular smooth muscle cell calcification via DUSP1-dependent p38 MAPK inhibition

Figure 6. Effects of dual-specificity phosphatase 1 knockdown on anti-calcifying properties of fisetin in VSMCs. Relative mRNA expression (n=10) of DUSP1 (A), BMP2 (B), CBFA1 (C), ALPL (D) and CDKN1A (E) in HAoSMCs transfected for 72h with negative control (Neg.si) or DUSP1 (DUSP1si) siRNA and treated for 48h with control (CTR) or calcification medium (Calc.) without and with 1 μM fisetin (Fis). **(p<0.01), ***(p<0.001) significant vs. Neg.si group; †(p<0.05), ††(p<0.01) significant vs. Neg.si+Calc.-treated group; ‡‡(p<0.01), ‡‡‡(p<0.001) significant between Neg.si+Calc.+Fis- and DUSP1si+Calc.+Fis-treated groups.