Research Paper Advance Articles
Fisetin ameliorates vascular smooth muscle cell calcification via DUSP1-dependent p38 MAPK inhibition
Figure 7. Effects of dual-specificity phosphatase 1 knockdown on the protective role of fisetin during VSMC calcification. Calcification detected by Osteosense fluorescence imaging (A) and normalized calcium content (n=7, B) in HAoSMCs transfected with negative control (Neg.si) or DUSP1 (DUSP1si) siRNA and treated for 11d with control (CTR) or calcification medium (Calc.) without and with 1 μM fisetin (Fis). Calcified areas: white pseudocolor. *(p<0.05), ***(p<0.001) significant vs. Neg.si group; †(p<0.05), ††(p<0.01) significant vs. Neg.si+Calc.-treated group; ‡‡‡(p<0.001) significant between Neg.si+Calc.+Fis- and DUSP1si+Calc.+Fis-treated groups.