Research Paper Volume 17, Issue 5 pp 1275—1297

Figure 6. Gene expression profiling of skeletal muscle using RNA sequencing analysis. (A) The RNA expression pattern analysis in skeletal muscle (femoris) using Principal Component Analysis (PCA). A total of 5977 genes were involved in this analysis. Two samples in each group and genders were used. Mouse age, 5 weeks old. (B) Analysis of differently expressed genes (DEGs) between different groups. 155 genes were observed in both groups, DKO vs. WT and Cisd2 KO vs. WT. (C) IPA analysis of gene expression in DKO muscles alone (502 genes). (D) The IPA analysis of gene expression in both of DKO and Cisd2 KO muscles (155 genes). The X axis represents negative log p-values based on the probability that genes in the uploaded dataset were included in the predefined IPA canonical pathways. Pathways with green words were the pathways which were found in both analyses in (C, D).