Bleomycin promotes cellular senescence and activation of the cGAS-STING pathway without direct effect on fibrosis in an idiopathic pulmonary fibrosis model

Yoko Miura; Nadia Milad; Akiho Takata; Satoshi Kanazawa

doi:doi:10.18632/aging.206312

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Research Paper Advance Articles

Bleomycin promotes cellular senescence and activation of the cGAS-STING pathway without direct effect on fibrosis in an idiopathic pulmonary fibrosis model

Figure 1. Lungs with iUIP were already in senescence state. Total RNA was extracted from iUIP, normal mice (healthy control mice with the same genetic background as iUIP mice), and age-matched control lungs. P16^INK4A (A), P19^ARF (B), Mmp3 (C), Mmp7 (D), Mmp8 (E), Mmp9 (F), Mmp10 (G), Mmp12 (H), Spp1 (I), Ccl20 (J), Cxcl5 (K), Cxcr1 (L), Il6 (M), S100a8 (N), and Adam17 (O) expression as determined by qPCR. Hprt served as an internal control. The results are shown as mean ± SE of five mice at normal and iUIP and four mice at age-matched control. Asterisks indicate ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, and ^****P < 0.0001 compared with normal lungs. “ns”, not statistically significant.