Research Paper Advance Articles

Figure 1. Conditional upregulation of Runx1 expression in Krt19CreERT; Rosa26-Runx1 mice following Tamoxifen Injections. (A) The gene expression of Runx1 was increased in the NP of control mice at 10M compared to 5M. n = 4~5 mice/group. Student’s t-test was performed. * p < 0.05. (B) Generation of double transgenic Krt19CreERT; Rosa26-Runx1 mice. Upon tamoxifen injection, Runx1 was specifically overexpressed in the NP tissues. (C) Tamoxifen was administered intraperitoneally at 4 weeks of age. In the Tam X2 group, tamoxifen was injected once a day for two consecutive days while in the Tam X3 group, it was injected once a day for three consecutive days. Runx1 overexpression was verified by Q-PCR using RNAs isolated from NP tissues collected seven days after the final injection in each group. For X-ray and histological analyses, mice were sacrificed at 5, 7, and 10 months (M) of age. (D) Quantitative PCR analysis revealed a significant upregulation in the gene expression of Runx1 in Tam X2 and Tam X3 groups compared to the control. n = 5 mice/group. One-way ANOVA followed by Tukey’s post-test was performed. (*** p < 0.001, * p < 0.05). Tam X2 refers to Krt19CreERT; Rosa26-Runx1 mice injected with tamoxifen for two consecutive days and Tam X3 refers to Krt19CreERT; Rosa26-Runx1 injected with tamoxifen for three consecutive days.