Research Paper Advance Articles

Figure 4. Proteomic analysis of Tg2576 mice infused with blood from old and young wild type mice. (A) Heatmap of quantifiable proteins from the Old Blood and Young Blood groups, showing log fold-change (logFC) in protein expression. (B) Venn diagram of differentially expressed proteins in an Old Blood vs. Young Blood groups comparison. (C) Canonical pathway analysis of differentially expressed proteins, highlighting enriched signaling pathways with activation z-scores. (D–F) Differentially expressed proteins in cAMP-mediated signaling, synaptogenesis signaling, and endocannabinoid neuronal synapse pathways, respectively. Gene names are used. (G, H) Representative western blot image and quantitative analysis of expression of CACNA2D2 in brain homogenates. (I, J) Representative western blot image and quantitative analysis of expression of BRAF in brain homogenates. (K, L) Representative western blot image and quantitative analysis of expression of Syngap1 in brain homogenates. (M, N) Representative western blot image and quantitative analysis of expression of MAPK9 in brain homogenates. (O, P) Representative western blot image and quantitative analysis of expression of GRK2 in brain homogenates. N = 3/group for proteomic analysis, and n = 4/group for protein validation (random mix of males and females; young donor group: 1–2M/2F; old donor group: 1–2M/2F). Data are expressed as mean ± SEM. Data in (H), (J), (L), (N), and (P) were analyzed using Student’s t-test. ^*p < 0.05.