Figure 2. Comparative transcriptomic analysis of GH overexpression and liver aging. Differential gene expression profiles from bGH-Tg versus WT mice were compared with those from aged (24-month-old) versus young (10-month-old) WT mice to identify overlapping molecular signatures of GH-induced and age-associated changes. (A) Venn diagram showing 596 common differentially expressed genes (DEGs) shared between GH overexpression (Tg vs WT) and aging (Old vs Young). (B) Correlation analysis of shared DEGs reveals a significant positive correlation (r = 0.30, p < 0.0001), with 394 genes upregulated and 65 genes downregulated in both conditions. (C) Pathway enrichment analysis of shared downregulated genes demonstrates enrichment of key metabolic pathways. (D) TRRUST analysis of shared downregulated genes identifies Hnf1a as a major regulatory transcription factor. (E) Pathway enrichment analysis of shared upregulated genes highlights enrichment of key gene ontology pathways. (F) TRRUST analysis of upregulated shared genes implicates transcription factors including Rfxap, Stat1, Irf1, Nfkb1, Rel, and other immune regulators as key drivers of the inflammatory transcriptional program common to both GH overexpression and aging.