Figure 1. Brain region-specific overexpression of endogenous and human SNCA in rats. (A) Schematic illustration showing the experimental design of 5- and 12-months-old wildtype (WT) and transgenic (TG) BAC SNCA rats. RNA was isolated from striatum, cortex and cerebellum for 5 animals per group and used for RNA-seq to identify differential expression. (B) Expression level of endogenous rat Snca (left) and human SNCA (right) as individual nRPKM data points per rat across experimental groups with mean and standard error of the mean. (C) Composition and expression level of endogenous and human SNCA transcript isoforms across experimental groups.