Search
To search the journal, enter a term in the search bar. If you'd like to find specific authors, titles, or abstracts, use the advanced search to the right.
Search Results
2 results found. Results per page: [ 20 ][ 40 ][ 60 ][ 80 ][ 100 ][ 200 ][ 300 ]
Sort by: [ Publication Date ][ Score ]
Year of publication: [ 2025 ][ 2024 ][ 2023 ][ 2022 ][ 2021 ][ 2020 ][ 2019 ][ 2018 ][ 2017 ][ 2016 ][ 2015 ][ 2014 ][ 2013 ][ 2012 ][ 2011 ][ 2010 ][ 2009 ][ Any ]
-
Research Paper Volume 13, Issue 14 pp 18718-18739
Endothelial microparticle-associated protein disulfide isomerase increases platelet activation in diabetic coronary heart disease
Relevance score: 17.116928Xiao-Di Sun, Lu Han, Hong-Tao Lan, Ran-Ran Qin, Ming Song, Wei Zhang, Ming Zhong, Zhi-Hao Wang
Keywords: protein disulfide isomerase, endothelial microparticle, platelet activation, GPIIb/IIIa, diabetes
Published in Aging on July 20, 2021
-
Research Paper Volume 11, Issue 16 pp 6358-6370
Platelet activation in diabetic mice models: the role of vascular endothelial cell-derived protein disulfide isomerase-mediated GP IIb/IIIa receptor activation
Relevance score: 14.843367Ran-Ran Qin, Hui Zhu, Feng Wang, Ming Song, Pei-Lin Lin, Yan-Qiu Xing, Wei Zhang, Ming Zhong, Zhi-Hao Wang
Keywords: diabetes mellitus, protein disulfide isomerase, platelet activation, endothelial microparticle, glycoprotein IIb/IIIa receptor
Published in Aging on August 22, 2019
Results of the glucose tolerance test in ApoE-/- mice. (A) Comparison of IPGTT in two groups of mice at four weeks; (B) Comparison of IPGTT before and after feeding in the normal diet group; (C) Comparison of IPGTT before and after feeding in the diabetic group. *P < 0.05, **P < 0.01, DM (10W) versus DM(4W); (D) The IPGTT results were compared between the two groups at 10 weeks. #P < 0.05, ##P < 0.01, ###P < 0.001, Chow (10W) versus DM (10W). Chow, normal diet group; DM, diabetic group (n = 10–30). Data were analyzed using one-way ANOVA.
ApoE-/- body weight variation. Measurement of the body weight of mice, *P < 0.05, versus Chow, #P < 0.05, versus Chow + Rutin. Chow, normal group; Chow + Rutin, normal diet plus rutin; DM, Diabetic group; DM + Rutin, diabetic group plus rutin (n = 4–19). Data were analyzed using one-way ANOVA.
Isolation and identification of microparticles from endothelial cells. CD144 was used to mark endothelial cell-derived microparticles (EMP). (A) The positive rate of CD144 in the plasma before isolation; (B) The positive rate of CD144 in the plasma after isolation; (C) The isolated microparticles were further selected, using FCM, with the standards of 100 nm and 1000 nm; (D) Transmission electron microscopy was used to observe the obtained microparticles with a diameter >100 nm.
PDI was carried by EMPs, and the contents of CD144-positive EMPs and EMP-PDI in the plasma were increased in the diabetic group. (A) The content of CD144-positive EMPs in the plasma of diabetic mice was increased; (B) Dual-color flow cytometry was used to show that PDI is carried by the particles in endothelial cells; (C) The content of EMP-PDI in the plasma of diabetic mice was increased;*P < 0.05, **P < 0.01, ***P < 0.001 versus Chow; #P < 0.05, ##P < 0.01, ###P < 0.001 versus Chow + Rutin; †P < 0.05, ††P < 0.01, ‡P < 0.001 versus DM. Chow, normal group; Chow + Rutin, normal diet plus rutin; DM, Diabetic group; DM + Rutin, diabetic group plus rutin (n = 5–11). Data were analyzed using one-way ANOVA.
The plasma content of VWF and PDI was detected and the activity of platelet between the four groups was compared. (A) The VWF content in the plasma was detected using ELISA; (B) The PDI content in the plasma was detected using ELISA; (C) Flow cytometry was used to detect activated GP IIb/IIIa on the platelet surface; (D) Flow cytometry was used to detect P-selectin expression on the platelet surface *P < 0.05, **P < 0.01, ***P < 0.001, versus Chow; #P < 0.05, ##P < 0.01, ###P < 0.001, versus Chow + Rutin, †P < 0.05, ††P < 0.01, ‡P < 0.001, versus DM. Chow, normal group; Chow + Rutin, normal diet plus rutin; DM, Diabetic group; DM + Rutin, diabetic group plus rutin (n = 3–8). Data were analyzed using one-way ANOVA.
Platelets of normal C57 mice were stimulated with EMP and pretreatment EMPs. Adenosine diphosphate (ADP) (10 μg/mL), RL90 (1 μg/mL), and rutin (60 μM) were used to pretreat EMPs obtained from four groups; then the EMP and the pretreatment EMP were used to stimulate the platelets of normal C57 mice, respectively. FCM was used to detect the level of platelet activation. EMP from the diabetic mice can significantly activate platelets, while RL90 and rutin could inhibit this process. Data were analyzed using one-way ANOVA. (A) Comparisons of the GP IIb/IIIa receptor expression level on the platelet surface of C57 mice stimulated using different pretreated EMPs, within each group. The GP IIb/IIIa receptor expression significantly increased in the EMP stimulus subgroup. *P < 0.05, **P < 0.01, ***P < 0.001 versus Control; #P < 0.05, ##P < 0.01, ###P < 0.001 versus EMP; P < 0.05, ††P < 0.01, ‡P < 0.001 versus EMP + ADP (n = 3–5). (B) Comparisons of the expression level of P-selectin on the platelet surface of C57 mice stimulated using different pretreated EMPs, within each group. The P-selectin expression was significantly increased in the EMP stimulus subgroup. *P < 0.05, **P < 0.01, ***P < 0.001 versus Control; #P < 0.05, ##P < 0.01, ###P < 0.001 versus EMP; †P < 0.05, ††P < 0.01, ‡P < 0.001 versus EMP + ADP (n = 3–10). (C) Comparisons of the GP IIb/IIIa receptor expression level on the platelet surface of normal C57 mice among the four groups stimulated using different pretreated EMP. *P < 0.05, **P < 0.01,***P < 0.001 versus Chow; #P < 0.05, ##P < 0.01, ###P < 0.001 versus Chow + Rutin; †P < 0.05, ††P < 0.01, ‡ P < 0.001 versus DM (n = 3–8). (D) Comparisons of the P-selectin expression level on the platelet surface of normal C57 mice among the four groups stimulated using different pretreated EMP. *P < 0.05, **P < 0.01, ***P < 0.001 versus Chow; #P < 0.05, ##P < 0.01, ###P < 0.001 versus Chow + Rutin; †P < 0.05, ††P < 0.01, ‡ P < 0.001 versus DM (n = 3–9). (E) The Duolink® in-situ proximity ligation assay was used to detect the GPIIb/IIIa receptor and EMP-PDI on the platelet surface. The red dot granule represents GPIIb/IIIa receptor binding to PDI (scale = 50 μm).